Research Laboratories
About the Research Laboratories
The Research Laboratories are a Core Facility under the Department of Dentistry and Oral Health (IOOS) established in 2014. The facility is available for all researchers at the department, but is also open to researchers from other departments at Aarhus University as well as other universities, regions, municipalities, hospitals and industries. Research collaborations are encouraged, and the laboratory staff will be helpful in establishing contact with the department's researchers.
The Research Laboratories are divided into specialized laboratories within microbiology, molecular biology, cell cultivation, materials research, histology and 3D printing. The laboratory employs five highly specialized laboratory technicians/medical laboratory technicians all with many years of experience within their particular field of expertise and in cooperating with internal and external researchers in terms of planning and carrying out scientific work.
The day-to-day management is handled by Associate Professor David Kraft, who all interested parties are welcome to contact.
Microbiology
- Bacterial and fungal cultivation, risk groups I and II (aerobic and anaerobic, CO2, nitrogen)
- Identification of bacteria by cultivating them in/on selective media
- Media and reagents preparation
- Access to freeze-drying bacteria and storage in -80o freezer
- Growth curves of bacteria via spectrophotometrical measurements
- Resistance determination of bacteria
- Biochemical tests of bacteria
- Biofilm tests
- Pathological “fungus scrape” by PAS colouring
- Water analyses, in-house sampling and testing of the quality of water in the dental units, untreated and treated water
- Bacterial adhesion tests in flow-cells by confocal microscopy
- pH testing in the extra cellular matrix
Molecular biology
- Purification of DNA from gram-positive and gram-negative bacteria and fungi
- PCR
- RT-PCR
Dental material research and chemical analysis
Chemistry:
- measuring aniones such as phosphate, chloride, nitrate, sulphate and fluoride in various materials such as toothpaste, saliva, soft drinks etc.
- measuring cations such as calcium, magnesium and potassium
- measuring with an ion-chromatograph and ion selective electrodes
- pH
Dental cement:
- ISO standard methods for measuring dental cement
- cementation time
- thickness of film
- humidity, absorption of humidity
- fluoride emission
- radiopacity
INSTRON®3366 unit for testing dental materials
- compression strength of various dental materials
- elasticity of dental materials
- adhesive strength such as dental bands, crowns, posts, orthodontic wire etc.
Histology
- Sectioning in hard and soft tissue
- Sectioning paraffin sections in soft or decalcified tissue (2-7 µm)
- Sectioning plastic sections in undecalcified hard tissue (including radiographs) (4-9 µm)
- Saw sectioning in undecalcified hard tissue with and without implants (different units each with their own benefit). Sectioning (down to 50 µm)
- Colouring of sections (routine colouring, specialized colouring)
- Statistics of measured data
- Cutting tissue blocks
Molecular histology
- Making saw sections of extracted teeth
- Sectioning above-mentioned sections (to <100 µm)
- Microradiography of the sections
- Embedding and sectioning plastic embedded tissue (mainly soft or decalcified tissue) – 1-2 µm, perhaps thinner
- Radiographs used for control of decalcification degree of the tissue
- Preparation for Scanning Electron Microscopy (SEM)
- Preparation for Transmission Electron Microscopy (TEM)
- Fluorescence-in-situ hybridization (FISH)
- Immune colouring
Microscopy and imaging
- Light and fluorescence microscopy
- Microscoping sections, histomorphometrics
- High-quality photography - macroscopic, microscopic
- Imaging, setting photos and graphs for articles
Other services
- Support for the clinics of the department, for example sterile balancing, dilution
- Disposal of the chemical waste and risk waste of the department
- Collecting, handling and storing saliva
- Innovation and optimizing
- Blood tests
- Assistance in animal trials
- Data treatment in connection with trials
- Renovation and maintenance of the specimens at the Anatomical museum
- Maintenance of equipment as well as administration and updating of workplace guidelines for dangerous substances and materials used by dental students and dental technician students at the skills laboratories
- Quarterly control of alarm and ventilation functions of fume cupboards at the department
Fees
The Research Laboratories are accessible for external and internal projects under the following conditions:
- Internal IOOS projects
- You will be charged for materials. At the Research Laboratories, we recommend that researchers at the department make project applications in cooperation with the Research Laboratories, including budgets for work to be carried out in the laboratories.
- Research cooperation
- You will be charged for materials. Other costs are agreed on in collaboration with involved department researchers.
- External projects
- The price is calculated according to the university guidelines.
Contact David Kraft for a price estimate.
Booking
If you are interested in using the facilities of the Research Laboratories, please write a short description of your project and the kind of help you require. Send this to
David Kraft dck@dent.au.dk
Alternatively, contact an employee for further information.
Equipment
- Ion chromatograph
- Material test equipment, INSTRON 3366
- Autoclave, Laboklav 55MSX
- Centrifuge Sorvall ST 8R & Eppendorf 5810Spectrofotometer, DeNovix DS11 & V-3000PC
- Aria MX real time PCR system
- Nucleocounter
- Tissuelyser II
- Laf bench, Nordic Safe Esco Class II BSC
- CO2 incubator, Binder & Thermo scientific Midi 40
- -80°C freezer, Forma 900 series
- Plate reader, EL800
- Colony counter, Interscience Scan 100
- Inverteret Olympus mikroskop with DP72 camera
- Edwards coating system E306A (coalcoater)
- Bio-Rad SEM coating system (sputtercoater)
Microtomes
- Reichert Jung polycut E (uncalcifies blocks)
- Leica RM 2255 (small uncalcifies blocks + descale blocks)
- Reichert Jung 2050 - ( descale blocks)
- Reichert Jung ultracut E (ultramicrotome)
Saw for hard tissue & implants
- Leiden saw - meprotech
- Exakt saw
- Leitz 1600 - embedded and unembedded tissue
Grinder
- Exakt grinder
- Struers knuth-rotor-2
Articles
2020
Schlafer, S. & Frost Kristensen, M. (2020). Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy. Journal of visualized experiments : JoVE, (155). https://doi.org/10.3791/60270
2019
Kruse, C., Spin-Neto, R., Kraft, D. C. E., Væth, M. & Kirkevang, L-L. (2019). Diagnostic accuracy of cone beam computed tomography used for diagnosis of apical periodontitis using histopathology of ex-vivo human jaws as reference standard. Abstract from 19th Biennial ESE Congress, Wien, Austria. https://www.e-s-e.eu/our-events/past-biennial-congresses/
Pagh, S., Pertoldi, C., Petersen, H. H., Jensen, T. H., Hansen, M. S., Madsen, S., Kraft, D. C. E., Iversen, N., Roslev, P. & Chriel, M. (2019). Methods for the identification of farm escapees in feral mink (Neovison vison) populations. PLOS ONE, 14(11), [0224559]. https://doi.org/10.1371/journal.pone.0224559
Bizelli-Silveira, C., Abildtrup, L. A., Spin-Neto, R., Foss, M., Søballe, K. & Kraft, D. C. E. (2019). Strontium enhances proliferation and osteogenic behavior of bone marrow stromal cells of mesenchymal and ectomesenchymal origins in vitro. Clinical and Experimental Dental Research, 5(5), 541-550. https://doi.org/10.1002/cre2.221
Dehli, J., Karlsson, C., Bizelli-Silveira, C., Jiang, X., Kraft, D. & Foss, M. (2019). E-cadherin mediated cell-biomaterial interaction reduces migration of keratinocytes in-vitro. Colloids and Surfaces B: Biointerfaces, 180, 326-333. https://doi.org/10.1016/j.colsurfb.2019.04.010
Dige, I. & Nyvad, B. (2019). Candida species in intact in vivo biofilm from carious lesions. Archives of Oral Biology, 101, 142-146. https://doi.org/10.1016/j.archoralbio.2019.03.017
Kruse, C., Spin-Neto, R., Kraft, D. C. E., Vaeth, M. & Kirkevang, L-L. (2019). Diagnostic accuracy of cone beam computed tomography used for assessment of apical periodontitis: an ex vivo histopathological study on human cadavers. International Endodontic Journal, 52(4), 439-450. https://doi.org/10.1111/iej.13020
2018
Bizelli-Silveira, C., Pullisaar, H., Abildtrup, L. A., Andersen, O. Z., Spin-Neto, R., Foss, M. & Kraft, D. C. E. (2018). Strontium enhances proliferation and osteogenic behavior of periodontal ligament cells in vitro. Journal of Periodontal Research, 53(6), 1020-1028. https://doi.org/10.1111/jre.12601
Schlafer, S., Baelum, V. & Dige, I. (2018). Improved pH-ratiometry for the three-dimensional mapping of pH microenvironments in biofilms under flow conditions. Journal of Microbiological Methods, 152, 194-200. https://doi.org/10.1016/j.mimet.2018.08.007
Schlafer, S., Garcia, J., Meyer, R. L., Vaeth, M. & Neuhaus, K. W. (2018). Effect of DNase treatment on adhesion and early biofilm formation of Enterococcus faecalis. European Endodontic Journal, 3(2), 82-86. https://doi.org/10.14744/eej.2018.55264
Garcia, J., Kamp, A. & Schlafer, S. (2018). The use of pH ratiometry to measure extracellular pH in biofilms of Candida spp. 485-485. Poster session presented at 65th Congress of the European Organisation for Caries Research, København, Denmark. https://doi.org/10.1159/000488302
Schlafer, S., Kamp, A. & Garcia, J. (2018). A confocal microscopy based method to monitor extracellular pH in fungal biofilms. FEMS Yeast Research, 18(5), [049]. https://doi.org/10.1093/femsyr/foy049
Ranjkesh, B., Isidor, F., Kraft, D. C. E. & Løvschall, H. (2018). In vitro cytotoxic evaluation of novel fast-setting calcium silicate cement compositions and dental materials using colorimetric methyl-thiazolyl-tetrazolium assay. Journal of Oral Science, 60(1), 82-88. https://doi.org/10.2334/josnusd.16-0751
Prabha, R. D., Kraft, D. C. E., Harkness, L., Melsen, B., Varma, H., Nair, P. D., Kjems, J. & Kassem, M. (2018). Bioactive Nano-fibrous Scaffold for Vascularized Craniofacial Bone Regeneration. Journal of Tissue Engineering and Regenerative Medicine, 12(3), e1537-e1548. https://doi.org/10.1002/term.2579
Hoedke, D., Enseleit, C., Gruner, D., Dommisch, H., Schlafer, S., Dige, I. & Bitter, K. (2018). Effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo. International Endodontic Journal, 51(S1), e23-e34. https://doi.org/10.1111/iej.12763
2017
Bizelli Silveira, C., Pullisaar, H., Foss, M., Spin-Neto, R. & Kraft, D. C. E. (2017). Strontium enhances periodontal ligament cell growth and osteogenic behaviour in vitro. e54 - 098. Abstract from EOS, 93rd Congress of the European Orthodontic Society, Montreux, Switzerland. http://www.eos2017.ch/
Dige, I., Spin-Neto, R. & Kraft, D. C. E. (2017). Bioimaging of teeth and their surrounding tissues and biofilm. Poster session presented at Danish Bioimaging Scientific Event, København, Denmark.
Schlafer, S., Meyer, R. L., Dige, I. & Regina, V. R. (2017). Extracellular DNA Contributes to Dental Biofilm Stability. Caries Research, 51, 436-442. https://doi.org/10.1159/000477447
Bizelli Silveira, C., Pullisaar, H., Foss, M., Spin-Neto, R. & Kraft, D. C. E. (2017). Strontium enhances periodontal ligament cell growth and osteogenic behaviour in vitro. SP55. Poster session presented at EOS, 93rd Congress of the European Orthodontic Society, Montreux, Switzerland. http://www.eos2017.ch/
2016
Jensen, J., Tvedesøe, C., Rölfing, J. H. D., Foldager, C. B., Lysdahl, H., Kraft, D. C. E., Chen, M., Baas, J., Le, D. Q. S. & Bünger, C. E. (2016). Dental pulp-derived stromal cells exhibit a higher osteogenic potency than bone marrow-derived stromal cells in vitro and in a porcine critical-size bone defect model. Sicot-J, 2, 16. https://doi.org/10.1051/sicotj/2016004
Schlafer, S. & Dige, I. (2016). Ratiometric Imaging of Extracellular pH in Dental Biofilms. Journal of Visualized Experiments, e53622. https://doi.org/10.3791/53622
2015
Prabha, R., Kraft, D. C. E., Melsen, B., Varma, H., Nair, P. D., Kjems, J. & Kassem, M. (2015). Electrospun PVA-PCL-HAB scaffold for craniofacial bone regeneration. Tissue Engineering. Part A, 21(Supplement 1), S-58. https://doi.org/10.1089/ten.tea.2015.5000.abstracts
Amin, Y. Y. I., Runager, K., Simoes, F., Celiz, A., Taresco, V., Rossi, R., Enghild, J. J., Abildtrup, L. A., Kraft, D. C. E., Sutherland, D. S., Alexander, M. R., Foss, M. & Ogaki, R. (2015). Combinatorial Biomolecular Nanopatterning for High-Throughput Screening of Stem-Cell Behavior. Advanced Materials. https://doi.org/10.1002/adma.201504995
Schlafer, S., Garcia, J., Greve, M., Raarup, M. K., Nyvad, B. & Dige, I. (2015). Microscopic monitoring of extracellular pH in dental biofilms. Abstract from ORCA, Brussels, Belgium.
Nielsen, H. K., Garcia, J., Væth, M. & Schlafer, S. (2015). Comparison of Riboflavin and Toluidine Blue O as Photosensitizers for Photoactivated Disinfection on Endodontic and Periodontal Pathogens In Vitro. P L o S One, [10.1371/journal.pone.0140720]. https://doi.org/10.1371/journal.pone.0140720
Schlafer, S., Garcia, J., Rasmussen, M. G., Raarup, M. K., Nyvad, B. & Dige, I. (2015). Ratiometric imaging of extracellular pH in bacterial biofilms with C-SNARF-4. Applied and Environmental Microbiology, 81(4), 1267-1273. https://doi.org/10.1128/AEM.02831-14
