Research Laboratories

About the Research Laboratories

 

The Research Laboratories are a Core Facility under the Department of Dentistry and Oral Health (IOOS) established in 2014. The facility is available for all researchers at the department, but is also open to researchers from other departments at Aarhus University as well as other universities, regions, municipalities, hospitals and industries. Research collaborations are encouraged, and the laboratory staff will be helpful in establishing contact with the department's researchers.  

The Research Laboratories are divided into specialized laboratories within microbiology, cell cultivation, materials research, histology and 3D printing. The laboratory employs five highly specialized laboratory technicians/medical laboratory technicians all with many years of experience within their particular field of expertise and in cooperating with internal and external researchers in terms of planning and carrying out scientific work.

The day-to-day management is handled by Associate Professor David Kraft, who all interested parties are welcome to contact.

Laboratory Services


Microbiology

  • Bacterial and fungal cultivation, risk groups I and II (aerobic and anaerobic, CO2, nitrogen)
  • Identification of bacteria by cultivating them in/on selective media
  • Media and reagents preparation
  • Access to freeze-drying bacteria and storage in -80o freezer
  • Growth curves of bacteria via spectrophotometrical measurements
  • Resistance determination of bacteria
  • Biochemical tests of bacteria
  • Biofilm tests
  • Pathological “fungus scrape” by PAS colouring
  • Water analyses, in-house sampling and testing of the quality of water in the dental units, untreated and treated water
  • Bacterial adhesion tests in flow-cells by confocal microscopy
  • pH testing in the extra cellular matrix

Cell cultivation

  • Cell cultivation
  • ELISA
  • SDS-PAGE
  • Western Blot

Molecular biology

  • Purification of DNA from gram-positive and gram-negative bacteria and fungi
  • PCR
  • RT-PCR

Dental material research and chemical analysis

Chemistry:

  • measuring aniones such as phosphate, chloride, nitrate, sulphate and fluoride in various materials such as toothpaste, saliva, soft drinks etc.
  • measuring cations such as calcium, magnesium and potassium
  • measuring with an ion-chromatograph and ion selective electrodes
  • pH

Dental cement:

  • ISO standard methods for measuring dental cement
  • cementation time
  • thickness of film
  • humidity, absorption of humidity
  • fluoride emission
  • radiopacity

INSTRON®3366 unit for testing dental materials

  • compression strength of various dental materials
  • elasticity of dental materials
  • adhesive strength such as dental bands, crowns, posts, orthodontic wire etc.

Histology

  • Sectioning in hard and soft tissue
  • Sectioning paraffin sections in soft or decalcified tissue (2-7 µm)
  • Sectioning plastic sections in undecalcified hard tissue (including radiographs) (4-9 µm)
  • Saw sectioning in undecalcified hard tissue with and without implants (different units each with their own benefit). Sectioning (down to 50 µm)
  • Colouring of sections (routine colouring, specialized colouring)
  • Statistics of measured data
  • Cutting tissue blocks

Molecular histology

  • Making saw sections of extracted teeth
  • Sectioning above-mentioned sections (to <100 µm)
  • Microradiography of the sections
  • Casting and sectioning plastic cast tissue (mainly soft or decalcified tissue) – 1-2 µm, perhaps thinner
  • Radiographs used for control of decalcification degree of the tissue
  • Preparation for Scanning Electromicroscopy (SEM)
  • Preparation for Transmisisonelectromicroscopy (TEM)
  • Fluorescence-in-situ hybridization (FISH)
  • Immune colourings

3D print

  • Design and print for research, for example Flow cells

Microscopy and imaging

  • Light and fluorescence microscopy
  • Microscoping sections, histomorphometrics
  • High-quality photography - macroscopic, microscopic
  • Imaging, setting photos and graphs for articles

Other services

  • Support for the clinics of the department, for example sterile balancing, dilution
  • Disposal of the chemical waste and risk waste of the department
  • Collecting, handling and storing saliva
  • Innovation and optimizing
  • Blood tests
  • Assistance in animal trials
  • Data treatment in connection with trials
  • Renovation and maintenance of the specimens at the Anatomical museum
  • Maintenance of equipment as well as administration and updating of workplace guidelines for dangerous substances and materials used by dental students and dental technician students at the skills laboratories
  • Quarterly control of alarm and ventilation functions of fume cupboards at the department

Practical Information


Fees

The Research Laboratories are accessible for external and internal projects under the following conditions:

  • Internal IOOS projects
    • You will be charged for materials. At the Research Laboratories, we recommend that researchers at the department make project applications in cooperation with the Research Laboratories, including budgets for work to be carried out in the laboratories.
  • Research cooperation
    • You will be charged for materials. Other costs are agreed on in collaboration with involved department researchers.
  • External projects
    • The price is calculated according to the university guidelines.

Contact David Kraft for a price estimate.

Booking

If you are interested in using the facilities of the Research Laboratories, please write a short description of your project and the kind of help you require. Send this to

David Kraft dck@dent.au.dk

Alternatively, contact an employee for further information.

Equipment

  • Ion chromatograph  
  • Material test equipment, INSTRON 3366
  • Autoclave, Laboklav 55MSX
  • Centrifuge Sorvall ST 8R & Eppendorf 5810Spectrofotometer, DeNovix DS11 & V-3000PC
  • Aria MX real time PCR system
  • Nucleocounter
  • Tissuelyser II
  • Laf bench, Nordic Safe Esco Class II BSC
  • CO2 incubator, Binder & Thermo scientific Midi 40
  • -80°C freezer, Forma 900 series 
  • Plate reader, EL800
  • Colony counter, Interscience Scan 100
  • Inverteret Olympus mikroskop with DP72 camera
  • Edwards coating system E306A (coalcoater)
  • Bio-Rad SEM coating system (sputtercoater)

Microtomes

  • Reichert Jung polycut E (uncalcifies blocks)
  • Leica RM 2255 (small uncalcifies blocks + descale blocks)
  • Reichert Jung 2050 - ( descale blocks)
  • Reichert Jung ultracut E (ultramicrotome)

Saw for hard tissue & implants

  • Leiden saw - meprotech
  • Exakt saw
  • Leitz 1600 - embedded and unembedded tissue

Grinder

  • Exakt grinder
  • Struers knuth-rotor-2

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